mattotto27141 mattotto27141
  • 01-06-2023
  • Chemistry
contestada

You have an aliquot of Coomassie Brilliant Blue R-250 dye with an unknown concentration in 20 mM Tris buffer, pH 7.5. This dye has a known extinction coefficient of 43,000 M-1 cm-1 at 555 nm. Using your UV-Vis spectrophotometers determine concentration of the dye stock.

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