You need to perform a serial dilution on a 1mL sample submitted to the lab. While you ordinarily make dilution tubes by adding 1mL to 9mL of solvent, you realize that you are limited by the volume of the original sample. You need to save at least half of the original sample for your analysis. Describe how you would create a serial dilution from 1:10 to 1:10,000, using only 500uL of the original sample solution.