Steps in cDNA synthesis
Order these steps used to generate a cDNA library from eukaryotic cells, beginning with the first step.
Rank the options below.
1) Digest mRNAs with RNase. Digest mRNAs with RNase.
2) Purify poly-adenylated mRNAs from eukaryotic cells.
3)Add oligo-dT primers.
4) Synthesize second strands with DNA polymerase and deoxyribonucleotides.
5) Cut hairpins with S1 nuclease. Cut hairpins with S1 nuclease. Open choices for matching
6) Denature cDNA-mRNA hybrids. Denature cDNA-mRNA hybrids.
7) Treat with reverse transcriptase and deoxynucleotides.
8) Ligate cDNAs into vectors.