Molarity = (Mass/ molar mass) x (1/ volume of solution in Litres)
Mass = Molarity x molar mass x volume of solution in Litres
Molarity of Tris = 100 mM = 0.1 M
volume of Tris sol. = 100 mL = 0.1 L
molar mass of Tris = 121.1 g/mol
Hence,
mass of Tris = Molarity of Tris x molar mass ofTris x volume of Tris solution
= 0.1 M x 121.1 g/mol x 0.1 L
= 1.211 g
mass of Tris = 1.211 g
