A protein has the amino acid sequence
DSRLSKTMYSIEAPAKLDWEQNMAL
How many peptide fragments would result from cleaving the sequence with either cyanogen bromide or trypsin? number of peptide fragments resulting from cleaving with cyanogen bromide? number of peptide fragments resulting from cleaving with trypsin?

Which of these reagents gives the smallest single fragment (in number of amino acid residues)?
(A) trypsin
(B) cyanotrypsin
(C) cyanogen bromide

Question

05-10-2019
Chemistry

contestada

A protein has the amino acid sequence
DSRLSKTMYSIEAPAKLDWEQNMAL
How many peptide fragments would result from cleaving the sequence with either cyanogen bromide or trypsin? number of peptide fragments resulting from cleaving with cyanogen bromide? number of peptide fragments resulting from cleaving with trypsin?

Which of these reagents gives the smallest single fragment (in number of amino acid residues)?
(A) trypsin
(B) cyanotrypsin
(C) cyanogen bromide

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rzamorauk rzamorauk · Answer 1 · 2019-10-09T03:34:42+02:00

Answer:

Number of peptide fragments resulting from cleaving with cyanogen bromide? A: Three peptide fragments

Number of peptide fragments resulting from cleaving with trypsin? A: Four peptide fragments

Which of these reagents gives the smallest single fragment (in number of amino acid residues)? A: CnBr, a dipeptide fragment consisting of AL (Alanine-Leucine)

Explanation:

Cyanogen bromide cleaves the methionine C-terminus, then we have a first fragment of 8 amino acids: DSRLSKTM, a second fragment of 15 aas YSIEAPAKLDWEQNM, and a last fragment of only 2 aas is produced, AL

Trypsin cuts the C-terminus of Arginine and Lysine, then we'll have a first fragment of 3 aas DSR, a second fragment consisting of also 3 aas LSK, a third fragment of 10 aas TMYSIEAPAK, and a last fragment of 9 aas LDWEQNMAL. All produced in three cut sites.