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The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by

1/V0= KM/(Vmax[S]) + 1/Vmax

To determine Km from a double-reciprocal plot, you would:

a. multiply the reciprocal of the x-axis intercept by –1.

b. multiply the reciprocal of the y-axis intercept by –1.

c. take the reciprocal of the x-axis intercept.

d. take the reciprocal of the y-axis intercept.

e. take the x-axis intercept, where V0= 1/2 Vmax.

Respuesta :

Answer:

option a

Explanation:

Lineweaver–Burk plot also known as double displacement plot is used for the study of enzyme kinetics.  

It is reciprocal of Michaelis-Menten equation. The Michaelis-Menten equation for enzyme catalysis is as follows:

[tex]V=\frac{V_{max} [S]}{K_m+[S]}[/tex]

Take the reciprocal

[tex]\frac{1}{V} =\frac{K_m+[S]}{V_{max}[S]} =\frac{K_m}{V_{max}} \frac{1}{[S]} +\frac{1}{V_{max}}[/tex]

The plot or graph between 1/V and 1/[S] is called Lineweaver–Burk plot.

Slope of the plot is [tex]\frac{K_m}{V_{max}}[/tex].  

Intercept of y-axis is .

Intercept of x-axis is [tex]-\frac{1}{K_m}[/tex]

Therefore, by taking the reciprocal of intercept of x-axis and multiplying by -1, Km value can be determined.

Therefore, the correct option is a.

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