A. incorrect type of diluent has been used
The Dilution approach is used to determine the minimal inhibitory concentration of an antimicrobial to inhibit or kill the bacteria/fungi and is the reference for antimicrobial susceptibility testing.
To obtain the preferred concentration, an easy dilution is one in which a unit volume of a liquid material of interest is blended with an adequate volume of a solvent liquid. the overall number of unit volumes wherein the material can be dissolved is the dilution component.
what are disc diffusion tests?
The disk diffusion test is a subculture-primarily based microbiology assay used in diagnostic and drug discovery laboratories. In diagnostic labs, the assay is used to determine the susceptibility of microorganisms isolated from a patient's infection to clinically permitted antibiotics.
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